Z-chromosome specific primers for chicken-quail hybrid blastoderm

Soichi Takagi; Tamao Ono; Akira Tsukada; Yusuke Atsumi; Shusei Mizushima; Noboru Saito; Kiyoshi Shimada

doi:10.2141/jpsa.44.209

Z-chromosome specific primers for chicken-quail hybrid blastoderm

Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	Journal of Poultry Science
Volume	44
Issue number	2
DOIs	https://doi.org/10.2141/jpsa.44.209
Publication status	Published - 2007
Externally published	Yes

Keywords

Chicken
PCR
PKCI
Quail
Z-chromosome

ASJC Scopus subject areas

Animal Science and Zoology

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10.2141/jpsa.44.209

Cite this

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title = "Z-chromosome specific primers for chicken-quail hybrid blastoderm",

abstract = "A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.",

keywords = "Chicken, PCR, PKCI, Quail, Z-chromosome",

author = "Soichi Takagi and Tamao Ono and Akira Tsukada and Yusuke Atsumi and Shusei Mizushima and Noboru Saito and Kiyoshi Shimada",

year = "2007",

doi = "10.2141/jpsa.44.209",

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T1 - Z-chromosome specific primers for chicken-quail hybrid blastoderm

AU - Takagi, Soichi

AU - Ono, Tamao

AU - Tsukada, Akira

AU - Atsumi, Yusuke

AU - Mizushima, Shusei

AU - Saito, Noboru

AU - Shimada, Kiyoshi

PY - 2007

Y1 - 2007

N2 - A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

AB - A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

KW - Chicken

KW - PCR

KW - PKCI

KW - Quail

KW - Z-chromosome

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DO - 10.2141/jpsa.44.209

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JO - Journal of Poultry Science

JF - Journal of Poultry Science

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ER -

Z-chromosome specific primers for chicken-quail hybrid blastoderm

Abstract

Keywords

ASJC Scopus subject areas

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