TY - JOUR
T1 - Zinc hydroxide induced respiratory burst in rat neutrophils
AU - Ogino, Keiki
AU - Izumi, Yukie
AU - Segawa, Hiroyuki
AU - Takeyama, Yuko
AU - Ishiyama, Hironobu
AU - Houbara, Tatsuya
AU - Uda, Taizo
AU - Yamashita, Shuji
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/1/3
Y1 - 1994/1/3
N2 - The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 μ/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 μM and 25 μM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodonapthalene-1-sulfonyl)-1H- hexahydro-1,4-diazepine, showed IC50 values ranging from 5 μM to 10 μM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.
AB - The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 μ/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 μM and 25 μM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodonapthalene-1-sulfonyl)-1H- hexahydro-1,4-diazepine, showed IC50 values ranging from 5 μM to 10 μM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.
KW - Neutrophil, rat
KW - Phagocytosis
KW - Respiratory burst
KW - Zinc hydroxide
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U2 - 10.1016/0926-6917(94)90082-5
DO - 10.1016/0926-6917(94)90082-5
M3 - Article
C2 - 8157083
AN - SCOPUS:0028177948
SN - 0926-6917
VL - 270
SP - 73
EP - 78
JO - European Journal of Pharmacology: Environmental Toxicology and
JF - European Journal of Pharmacology: Environmental Toxicology and
IS - 1
ER -