TY - JOUR
T1 - Zinc hydroxide stimulates superoxide production by rat alveolar macrophages
AU - Ogino, Keiki
AU - Izumi, Yukie
AU - Ishiyama, Hironobu
AU - Murata, Tomoko
AU - Kobayashi, Haruo
AU - Houbara, Tatsuya
PY - 1992/6/30
Y1 - 1992/6/30
N2 - The effect of zinc hydroxide on superoxide (O2-) production by rat alveolar macrophages was determined by chemiluminescence and by cytochrome c reduction. Zinc ions had no effect on the chemiluminescence of unstimulated alveolar macrophages. By contrast, zinc hydroxide (ZnOH2), a neutralized form of zinc ions, increased the chemiluminescence level and O2- release. Increased O2- release was inhibited by pertussis toxin, isoquinoline sulfonamide and pretreatment with EGTA. These findings indicate that zinc hydroxide formation from zinc compounds can stimulate the O2- production by alveolar macrophages by receptor-mediated and Ca2+-dependent process.
AB - The effect of zinc hydroxide on superoxide (O2-) production by rat alveolar macrophages was determined by chemiluminescence and by cytochrome c reduction. Zinc ions had no effect on the chemiluminescence of unstimulated alveolar macrophages. By contrast, zinc hydroxide (ZnOH2), a neutralized form of zinc ions, increased the chemiluminescence level and O2- release. Increased O2- release was inhibited by pertussis toxin, isoquinoline sulfonamide and pretreatment with EGTA. These findings indicate that zinc hydroxide formation from zinc compounds can stimulate the O2- production by alveolar macrophages by receptor-mediated and Ca2+-dependent process.
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U2 - 10.1016/0006-291X(92)91741-8
DO - 10.1016/0006-291X(92)91741-8
M3 - Article
C2 - 1320875
AN - SCOPUS:0026777023
SN - 0006-291X
VL - 185
SP - 1115
EP - 1121
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -