TY - JOUR
T1 - β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes
AU - Mizuno, Kenji
AU - Takahashi, Hideo Kohka
AU - Iwagaki, Hiromi
AU - Katsuno, Goutaro
AU - Kamurul, Huda A.S.M.
AU - Ohtani, Satoru
AU - Mori, Shuji
AU - Yoshino, Tadashi
AU - Nishibori, Masahiro
AU - Tanaka, Noriaki
PY - 2005/11/15
Y1 - 2005/11/15
N2 - We examined the effects of β2-adrenergic receptor (β2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that β2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective β2-AR antagonist, butoxamine. The selective β2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of β2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.
AB - We examined the effects of β2-adrenergic receptor (β2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that β2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective β2-AR antagonist, butoxamine. The selective β2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of β2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.
KW - Adrenergic receptor
KW - IL-12
KW - IL-18
UR - https://www.scopus.com/pages/publications/25444458849
UR - https://www.scopus.com/pages/publications/25444458849#tab=citedBy
U2 - 10.1016/j.imlet.2005.05.008
DO - 10.1016/j.imlet.2005.05.008
M3 - Article
C2 - 15998544
AN - SCOPUS:25444458849
SN - 0165-2478
VL - 101
SP - 168
EP - 172
JO - Immunology Letters
JF - Immunology Letters
IS - 2
ER -