A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kubo

研究成果

1 被引用数 (Scopus)

抄録

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

本文言語English
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ317-319
ページ数3
ISBN(印刷版)9780979806421
出版ステータスPublished - 2009
外部発表はい
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju
継続期間: 11月 1 200911月 5 2009

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
国/地域Korea, Republic of
CityJeju
Period11/1/0911/5/09

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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