A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata; Hiroyuki Michiue; Takashi Ohtsuki; Masaaki Miyazawa; Mizuki Kitamatsu

doi:10.1016/j.bmcl.2019.02.004

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata
, Hiroyuki Michiue
, Takashi Ohtsuki
, Masaaki Miyazawa
, Mizuki Kitamatsu

研究成果 › 査読

9 被引用数 (Scopus)

抄録

We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

本文言語	English
ページ（範囲）	878-881
ページ数	4
ジャーナル	Bioorganic and Medicinal Chemistry Letters
巻	29
号	7
DOI	https://doi.org/10.1016/j.bmcl.2019.02.004
出版ステータス	Published - 4月 1 2019

ASJC Scopus subject areas

生化学
分子医療
分子生物学
薬科学
創薬
臨床生化学
有機化学

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10.1016/j.bmcl.2019.02.004

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title = "A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus",

abstract = "We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.",

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AU - Hakata, Yoshiyuki

AU - Michiue, Hiroyuki

AU - Ohtsuki, Takashi

AU - Miyazawa, Masaaki

AU - Kitamatsu, Mizuki

PY - 2019/4/1

Y1 - 2019/4/1

N2 - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

AB - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

KW - Cell-penetrating peptide

KW - Drug delivery system

KW - Leucine zipper

KW - Nanog

KW - Peptide

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AN - SCOPUS:85060977862

SN - 0960-894X

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EP - 881

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 7

ER -

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

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