A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

Tomoka Takao; Daisuke Yamada; Takeshi Takarada

doi:10.1016/j.xpro.2022.101786

A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

Tomoka Takao
, Daisuke Yamada
, Takeshi Takarada

研究成果 › 査読

9 被引用数 (Scopus)

抄録

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1⁺ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).

本文言語	English
論文番号	101786
ジャーナル	STAR Protocols
巻	3
号	4
DOI	https://doi.org/10.1016/j.xpro.2022.101786
出版ステータス	Published - 12月 16 2022

ASJC Scopus subject areas

神経科学一般
生化学、遺伝学、分子生物学一般
免疫学および微生物学一般

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10.1016/j.xpro.2022.101786

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abstract = "Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).",

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AB - Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).

KW - Cell culture

KW - Cell differentiation

KW - Developmental biology

KW - Organoids

KW - Stem cells

KW - Tissue engineering

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A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

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