A rapid microfluidic switching system for analysis at the single cellular level

Akira Yamada, Yuki Katanosaka, Satoshi Mohri, Keiji Naruse

研究成果査読

12 被引用数 (Scopus)

抄録

Analysis of cellular responses to chemicals at high spatiotemporal resolution is required for precise understanding of intracellular signal transduction. Here, we demonstrated a novel method for applying different solutions to a part of or all of a cell at high spatiotemporal resolution. We fabricated a microfluidic device using polydimethylsiloxane, and the sharp interface between the two solution streams flowing in the channel was used for the application of different solutions. We constructed a computer-controlled system to control the interface movement precisely, rapidly, and reproducibly during positioning, and spatial and temporal resolutions attained were 1.6 μm and 189 ms, respectively. We then applied the present system to the analysis of intracellular responses to chemicals. We were able to measure [Ca 2+] increases within 500 ms, when one laminar stream covered a part of the cell. This method can be used as a generic platform to investigate responses against drugs at the single cell level.

本文言語English
論文番号5401110
ページ(範囲)306-311
ページ数6
ジャーナルIEEE Transactions on Nanobioscience
8
4
DOI
出版ステータスPublished - 12月 2009

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 電子工学および電気工学
  • バイオテクノロジー
  • 生体医工学
  • 医学(その他)
  • コンピュータ サイエンスの応用
  • 薬科学

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