Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.
|出版ステータス||Published - 9月 2005|
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