Abnormalities of glycogenes in tonsillar lymphocytes in IgA nephropathy

Tatsuyuki Inoue, Hitoshi Sugiyama, Masashi Kitagawa, Keiichi Takiue, Hiroshi Morinaga, Yoko Kikumoto, Yohei Maeshima, Kunihiro Fukushima, Kazunori Nishizaki, Hirofumi Akagi, Yoshiyuki Hiki, Hirofumi Makino

研究成果

12 被引用数 (Scopus)

抄録

Glycosylation, which represents the most complex post-translational modification, plays a pivotal role during protein maturation, and is orchestrated by numerous glycosyltransferases. Aberrant O-glycosylation of serum and tonsillar IgA1 is presumed to be one of the pathogeneses of IgA nephropathy (IgAN). However, the synthesis of underglycosylated IgA1 in tonsils has not yet been characterized. This study investigated tonsillar B lymphocytes of IgAN using tonsils from patients with chronic tonsillitis and sleep apnea syndrome. Gene expression of β1,3-galactosyltransferase (β3GalT), Cosmc, UDP-N-acetyl-α-D-galactosamine: polypeptide N-acetylgalactosaminyl- transferase 2, were significantly down regulated in tonsillar CD19-positive B lymphocytes from IgAN patients compared to control as determined by real-time RT-PCR. In contrast, the level of sialyltransferase was not significantly different among the three groups. Tonsillar B cell β3GalT gene expression significantly correlated with estimated GFR and negatively correlated with proteinuria and glomerular or interstitial injury score. Double immunofluorescent staining showed that some IgA-positive cells in the intrafollicular area were also positive for β3GalT staining. Western blotting showed the protein expression of β3GalT in the tonsils to significantly decrease in IgAN in comparison to the controls. These data suggest the downregulation of β3GalT in tonsillar B lymphocytes to be closely associated with the clinical characteristics of IgAN.

本文言語English
ホスト出版物のタイトルRecent Advances in Tonsils and Mucosal Barriers of the Upper Airways
ホスト出版物のサブタイトル7th International Symposium on Tonsils and Mucosal Barriers of the Upper Airways, Asahikawa, July 2010: Proceedings
編集者Yasuaki Harabuchi, Tatsuya Hayashi, Akihiro Katada
ページ71-74
ページ数4
DOI
出版ステータスPublished - 8月 2011

出版物シリーズ

名前Advances in Oto-Rhino-Laryngology
72
ISSN(印刷版)0065-3071

ASJC Scopus subject areas

  • 耳鼻咽喉科学

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