ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells

Teiichi Nishiki; Shuh Narumiya; Narito Morii; Masamitsu Yamamoto; Motohatsu Fujiwara; Yoichi Kamata; Genji Sakaguchi; Shunji Kozaki

doi:10.1016/0006-291X(90)91760-P

ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells

Teiichi Nishiki, Shuh Narumiya, Narito Morii, Masamitsu Yamamoto, Motohatsu Fujiwara, Yoichi Kamata, Genji Sakaguchi, Shunji Kozaki

大学院医歯薬学総合研究科

研究成果 › 査読

72 被引用数 (Scopus)

抄録

Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.

本文言語	English
ページ（範囲）	265-272
ページ数	8
ジャーナル	Biochemical and Biophysical Research Communications
巻	167
号	1
DOI	https://doi.org/10.1016/0006-291X(90)91760-P
出版ステータス	Published - 2月 28 1990

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

文献へのアクセス

10.1016/0006-291X(90)91760-P

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引用スタイル

Nishiki, T, Narumiya, S, Morii, N, Yamamoto, M, Fujiwara, M, Kamata, Y, Sakaguchi, G & Kozaki, S 1990, 'ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells', Biochemical and Biophysical Research Communications, vol. 167, no. 1, pp. 265-272. https://doi.org/10.1016/0006-291X(90)91760-P

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abstract = "Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.",

author = "Teiichi Nishiki and Shuh Narumiya and Narito Morii and Masamitsu Yamamoto and Motohatsu Fujiwara and Yoichi Kamata and Genji Sakaguchi and Shunji Kozaki",

note = "Funding Information: We thank Dr. S. Furukawa for useful discussions. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan, and grants from the Cell Science Foundation, the Takeda Science Founcla-tion, the Watanabe Memorial Fund, the Japan Intractable Diseases Foundation and the Japanese Foundation on Metabolism and Diseases.",

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AU - Sakaguchi, Genji

AU - Kozaki, Shunji

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