@inbook{e708f792cd914aceb051d2e2021086d0,
title = "Affinity labeling and purification of plant chitin-binding LysM receptor with chitin octasaccharide derivatives",
abstract = "Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds to N-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.",
keywords = "Affinity labeling, Affinity ligand, Biotinylation, CEBiP, Chitin oligosaccharide, Chitin receptor, LysM, Purification",
author = "Tomonori Shinya and Naoto Shibuya and Hanae Kaku",
note = "Funding Information: This article was supported by JSPS KAKENHI Grant Number JP18H02208 to H.K., JP18K05558 to T.S., and MEXT Supported Program for the Strategic Research Foundation at Private Universities 2014?2018 (S1411023) from MEXT, Japan, to H.K. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0430-4_39",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "401--412",
booktitle = "Methods in Molecular Biology",
}