An internal control for the rapid detection of mycobacteria by amplification of a segment of the gene encoding alpha antigen.

H. Kitaura, N. Ohara, T. Matsuo, T. Yamada

研究成果査読

抄録

The internal control of DNA for the rapid detection of mycobacteria by PCR is described. The 1100bp fragment for internal control was produced from Streptomyces lividans DNA with the primers used for the rapid detection of mycobacteria by PCR. The amplified reaction consequently produced two products with 782bp for mycobacteria and 1100bp for the internal control extracted from all mycobacterial DNAs containing internal control so far examined. The 1100bp amplified fragment proved to be useful as an internal control with the same primer-binding sequence for the detection of mycobacteria.

本文言語English
ページ(範囲)429-433
ページ数5
ジャーナルThe new microbiologica : official journal of the Italian Society for Medical, Odontoiatric, and Clinical Microbiology (SIMMOC)
18
4
出版ステータスPublished - 10月 1995
外部発表はい

ASJC Scopus subject areas

  • 医学一般

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