TY - JOUR
T1 - Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas
AU - Tsuji, Toshiya
AU - Nozaki, Isao
AU - Miyazaki, Masahiro
AU - Sakaguchi, Masakiyo
AU - Pu, Hong
AU - Hamazaki, Yusuke
AU - Iijima, Osamu
AU - Namba, Masayoshi
PY - 2001/11/23
Y1 - 2001/11/23
N2 - We recently reported the cloning of the REIC/Dkk-3 gene, whose expression was shown to be down-regulated in many human immortalized and tumor-derived cell lines [T. Tsuji et al. (2000) Biochem. Biophys. Res. Commun. 268, 20-24]. In the present study, we demonstrated that expression of the exogenous REIC/Dkk-3 gene in tumor cells inhibited cell growth. Furthermore, the level of REIC/Dkk-3 mRNA in normal human cells was lowest in the late G1 phase during the cell cycle. Then we found that the expression of REIC/Dkk-3 was significantly down-regulated in surgically resected non-small-cell lung carcinomas. We determined the REIC/Dkk-3 locus on chromosome 11p15, where loss of heterozygosity has frequently been observed in human tumors. These findings indicate that REIC/Dkk-3 may function as a tumor suppressor.
AB - We recently reported the cloning of the REIC/Dkk-3 gene, whose expression was shown to be down-regulated in many human immortalized and tumor-derived cell lines [T. Tsuji et al. (2000) Biochem. Biophys. Res. Commun. 268, 20-24]. In the present study, we demonstrated that expression of the exogenous REIC/Dkk-3 gene in tumor cells inhibited cell growth. Furthermore, the level of REIC/Dkk-3 mRNA in normal human cells was lowest in the late G1 phase during the cell cycle. Then we found that the expression of REIC/Dkk-3 was significantly down-regulated in surgically resected non-small-cell lung carcinomas. We determined the REIC/Dkk-3 locus on chromosome 11p15, where loss of heterozygosity has frequently been observed in human tumors. These findings indicate that REIC/Dkk-3 may function as a tumor suppressor.
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U2 - 10.1006/bbrc.2001.5972
DO - 10.1006/bbrc.2001.5972
M3 - Article
C2 - 11708809
AN - SCOPUS:0035940982
SN - 0006-291X
VL - 289
SP - 257
EP - 263
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -