TY - JOUR
T1 - Application Of Centrifugal Partition Chromatography To Tannins And Related Polyphenols
AU - Okuda, Takuo
AU - Yoshida, Takashi
AU - Hatano, Tsutomo
PY - 1988/9/1
Y1 - 1988/9/1
N2 - Centrifugal partition chromatography (CPC) has been applied to the separation and purification of bioactive polyphenols in extracts from an oriental crude drug, licorice, and also of oligomeric hydrolyzable tannins extracted from Heterocentron roseum. A tannin-like component, licochalcone B from Sinkiang licorice was separated from another component of related structure, licochalcone A, by normal-phase CPC using a solvent system, CHCl3-MeOH-H2O. A labile trimeric hydrolyzable tannin, nobotanin J which is readily hydrolyzed to a monomer and dimer(s) on a solid support upon gel-column chromatography, and a highly polar tetramer, nobotanin K were efficiently purified in the normal-phase and reversed-phase development, respectively.
AB - Centrifugal partition chromatography (CPC) has been applied to the separation and purification of bioactive polyphenols in extracts from an oriental crude drug, licorice, and also of oligomeric hydrolyzable tannins extracted from Heterocentron roseum. A tannin-like component, licochalcone B from Sinkiang licorice was separated from another component of related structure, licochalcone A, by normal-phase CPC using a solvent system, CHCl3-MeOH-H2O. A labile trimeric hydrolyzable tannin, nobotanin J which is readily hydrolyzed to a monomer and dimer(s) on a solid support upon gel-column chromatography, and a highly polar tetramer, nobotanin K were efficiently purified in the normal-phase and reversed-phase development, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0024067969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024067969&partnerID=8YFLogxK
U2 - 10.1080/01483918808076739
DO - 10.1080/01483918808076739
M3 - Article
AN - SCOPUS:0024067969
SN - 1082-6076
VL - 11
SP - 2447
EP - 2454
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 12
ER -