Artemisinin: An alternative treatment for oral squamous cell carcinoma

Eiki Yamachika, Temesgen Habte, Dolphine Oda

研究成果査読

48 被引用数 (Scopus)

抄録

Artemisinin (AR) is a widely used antimalarial drug. Recently, additional uses for AR as an anticancer drug were discovered. Using TUNEL, immunohistochemistry (IHS) markers and flow cytometry techniques, we evaluated the effect of AR and 5-FU on HPV 16 immortalized and transformed human gingival epithelial (IHGK) cells. The results of TUNEL showed that AR-treated IHGK cells consisted of 82% positive cells, while 5-FU-treated cells consisted of 18% positive cells. The IHS markers demonstrated positive staining with Bax, p53, CD40 and CD40L in AR-treated cells and negative staining with Bcl-2. 5-FU-treated cells demonstrated a profile similar to AR but with less intensity. Cell cycle by flow cytometry results showed that only 5-FU-treated cells demonstrated a significant S-phase rate increase to 45%. In conclusion, our results indicate that AR is cytotoxic to transformed oral epithelial cells through apoptosis, while 5-FU is cytotoxic primarily through cell toxicity.

本文言語English
ページ(範囲)2153-2160
ページ数8
ジャーナルAnticancer research
24
4
出版ステータスPublished - 7月 2004

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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