Asymmetric reduction of a variety of ketones with a recombinant carbonyl reductase: Identification of the gene encoding a versatile biocatalyst

Tadashi Ema; Hideo Yagasaki; Nobuyasu Okita; Kumiko Nishikawa; Toshinobu Korenaga; Takashi Sakai

doi:10.1016/j.tetasy.2005.02.004

Asymmetric reduction of a variety of ketones with a recombinant carbonyl reductase: Identification of the gene encoding a versatile biocatalyst

Tadashi Ema, Hideo Yagasaki, Nobuyasu Okita, Kumiko Nishikawa, Toshinobu Korenaga, Takashi Sakai

研究成果 › 査読

25 被引用数 (Scopus)

抄録

The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in E. coli. Recombinant E. coli co-producing SCR and GDH (glucose dehydrogenase) is an easy-to-use, synthetically useful biocatalyst, and 8 out of the 16 alcohols obtained had enantiomeric purities of >98% ee.

本文言語	English
ページ（範囲）	1075-1078
ページ数	4
ジャーナル	Tetrahedron Asymmetry
巻	16
号	6
DOI	https://doi.org/10.1016/j.tetasy.2005.02.004
出版ステータス	Published - 3月 21 2005

ASJC Scopus subject areas

触媒
物理化学および理論化学
有機化学
無機化学

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10.1016/j.tetasy.2005.02.004

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引用スタイル

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abstract = "The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in E. coli. Recombinant E. coli co-producing SCR and GDH (glucose dehydrogenase) is an easy-to-use, synthetically useful biocatalyst, and 8 out of the 16 alcohols obtained had enantiomeric purities of >98% ee.",

author = "Tadashi Ema and Hideo Yagasaki and Nobuyasu Okita and Kumiko Nishikawa and Toshinobu Korenaga and Takashi Sakai",

note = "Funding Information: We thank Dr. Hiroaki Yamamoto (Daicel Chemical Industries) and Prof. Tetsuo Toraya (Okayama University) for the determination of the internal amino-acid sequences of SCR and useful advice on gene expression, respectively. This work was supported by a Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science and by a grant from Venture Business Laboratory of Okayama University. We are grateful to the SC-NMR Laboratory of Okayama University for the measurement of NMR spectra.",

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AU - Nishikawa, Kumiko

AU - Korenaga, Toshinobu

AU - Sakai, Takashi

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