TY - JOUR
T1 - Cardiac progenitor cells from adult myocardium
T2 - Homing, differentiation, and fusion after infarction
AU - Oh, Hidemasa
AU - Bradfute, Steven B.
AU - Gallardo, Teresa D.
AU - Nakamura, Teruya
AU - Gaussin, Vinciane
AU - Mishina, Yuji
AU - Pocius, Jennifer
AU - Michael, Lloyd H.
AU - Behringer, Richard R.
AU - Garry, Daniel J.
AU - Entman, Mark L.
AU - Schneider, Michael D.
PY - 2003/10/14
Y1 - 2003/10/14
N2 - Potential repair by cell grafting or mobilizing endogenous cells holds particular attraction in heart disease, where the meager capacity for cardiomyocyte proliferation likely contributes to the irreversibility of heart failure. Whether cardiac progenitors exist in adult myocardium itself is unanswered, as is the question whether undifferentiated cardiac precursor cells merely fuse with preexisting myocytes. Here we report the existence of adult heart-derived cardiac progenitor cells expressing stem cell antigen-1. Initially, the cells express neither cardiac structural genes nor Nkx2.5 but differentiate in vitro in response to 5′-azacytidine, in part depending on Bmpr1a, a receptor for bone morphogenetic proteins. Given intravenously after ischemia/reperfusion, cardiac stem cell antigen 1 cells home to injured myocardium. By using a Cre/Lox donor/recipient pair (αMHC-Cre/R26R), differentiation was shown to occur roughly equally, with and without fusion to host cells.
AB - Potential repair by cell grafting or mobilizing endogenous cells holds particular attraction in heart disease, where the meager capacity for cardiomyocyte proliferation likely contributes to the irreversibility of heart failure. Whether cardiac progenitors exist in adult myocardium itself is unanswered, as is the question whether undifferentiated cardiac precursor cells merely fuse with preexisting myocytes. Here we report the existence of adult heart-derived cardiac progenitor cells expressing stem cell antigen-1. Initially, the cells express neither cardiac structural genes nor Nkx2.5 but differentiate in vitro in response to 5′-azacytidine, in part depending on Bmpr1a, a receptor for bone morphogenetic proteins. Given intravenously after ischemia/reperfusion, cardiac stem cell antigen 1 cells home to injured myocardium. By using a Cre/Lox donor/recipient pair (αMHC-Cre/R26R), differentiation was shown to occur roughly equally, with and without fusion to host cells.
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U2 - 10.1073/pnas.2132126100
DO - 10.1073/pnas.2132126100
M3 - Article
C2 - 14530411
AN - SCOPUS:0142027772
SN - 0027-8424
VL - 100
SP - 12313
EP - 12318
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -