@article{8a61ddb31beb4590b46f95e6171701e6,
title = "cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo",
abstract = "We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.",
keywords = "Chicken, Embryo, Transformer 2 (Tra 2), cDNA cloning",
author = "Ichiro Yamamoto and Akira Tsukada and Noboru Saito and Kiyoshi Shimada",
note = "Funding Information: We thank Dr. Craig A. Smith (Department of Paediatrics and Centre for Hormone Research, University of Melbourne, Royal Children's Hospital, Melbourne, Victoria, Australia) for teaching us the whole mount in situ hybridization technique. We also appreciate the Radioisotope Research Center of Nagoya University for use of the facilities. This study was supported by the Japanese Society for the Promotion of Science fellowship (01575) to I. Yamamoto.",
year = "2002",
month = dec,
day = "12",
doi = "10.1016/S0167-4781(02)00511-0",
language = "English",
volume = "1579",
pages = "185--188",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2-3",
}