Changes in structural features of free N-glycan and endoglycosidase activity during tomato fruit ripening

Kosuke Nakamura, Masami Inoue, Takeo Yoshiie, Katsutoshi Hosoi, Yoshinobu Kimura

研究成果査読

25 被引用数 (Scopus)

抄録

In developing plants, free N-glycans occur ubiquitously at micromolar concentrations. Such oligosaccharides have been proposed to be signaling molecules in plant development. As a part of a study to elucidate the physiological roles of de-N-glycosylation machinery involved in fruit ripening, we analyzed changes in the amounts and structural features of free N-glycans in tomato fruits at four ripening stages. The amount of high-mannose type free N-glycans increased significantly in accordance with fruit ripening, and the relative amounts of high-molecular size N-glycans, such as Man 8-9GlcNAc1, became predominant. These observations suggest that the de-N-glycosylation machinery, including endo-β-N- acetylglucosaminidase (ENGase) activity, is stimulated in the later stages of fruit ripening. But contrary to expectation, we found that total ENGase activities in the tomato fruits did not vary significantly with the ripening process, suggesting that ENGase activity must be maintained at a certain level, and that the expression of α-mannosidase involved in the clearance of free N-glycans decreases during tomato fruit ripening.

本文言語English
ページ(範囲)2936-2945
ページ数10
ジャーナルBioscience, Biotechnology and Biochemistry
72
11
DOI
出版ステータスPublished - 2008

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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