TY - JOUR
T1 - Characteristics of microbial communities in crustal fluids in a deep-sea hydrothermal field of the Suiyo Seamount
AU - Kato, Shingo
AU - Nakawake, Michiyuki
AU - Kita, Junko
AU - Yamanaka, Toshiro
AU - Utsumi, Motoo
AU - Okamura, Kei
AU - Ishibashi, Jun Ichiro
AU - Ohkuma, Moriya
AU - Yamagishi, Akihiko
PY - 2013
Y1 - 2013
N2 - To directly access the sub-seafloor microbial communities, seafloor drilling has been done in a deep-sea hydrothermal field of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific. In the present study, crustal fluids were collected from the boreholes, and the bacterial and archaeal communities in the fluids were investigated by culture-independent molecular analysis based on 16S rRNA gene sequences. Bottom seawater, sands, rocks, sulfide mound, and chimneys were also collected around the boreholes and analyzed for comparisons. Comprehensive analysis revealed the characteristics of the microbial community composition in the crustal fluids. Phylotypes closely related to cultured species, e.g., Alteromonas, Halomonas, Marinobacter, were relatively abundant in some crustal fluid samples, whereas the phylotypes related to Pelagibacter and the SUP05-group were relatively abundant in the seawater samples. Phylotypes related to other uncultured environmental clones in Alphaproteobacteria and Gammaproteobacteria were relatively abundant in the sand, rock, sulfide mound, and chimney samples. Furthermore, comparative analysis with previous studies of the Suiyo Seamount crustal fluids indicates the change in the microbial community composition for 3 years. Our results provide novel insights into the characteristics of the microbial communities in crustal fluids beneath a deep-sea hydrothermal field.
AB - To directly access the sub-seafloor microbial communities, seafloor drilling has been done in a deep-sea hydrothermal field of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific. In the present study, crustal fluids were collected from the boreholes, and the bacterial and archaeal communities in the fluids were investigated by culture-independent molecular analysis based on 16S rRNA gene sequences. Bottom seawater, sands, rocks, sulfide mound, and chimneys were also collected around the boreholes and analyzed for comparisons. Comprehensive analysis revealed the characteristics of the microbial community composition in the crustal fluids. Phylotypes closely related to cultured species, e.g., Alteromonas, Halomonas, Marinobacter, were relatively abundant in some crustal fluid samples, whereas the phylotypes related to Pelagibacter and the SUP05-group were relatively abundant in the seawater samples. Phylotypes related to other uncultured environmental clones in Alphaproteobacteria and Gammaproteobacteria were relatively abundant in the sand, rock, sulfide mound, and chimney samples. Furthermore, comparative analysis with previous studies of the Suiyo Seamount crustal fluids indicates the change in the microbial community composition for 3 years. Our results provide novel insights into the characteristics of the microbial communities in crustal fluids beneath a deep-sea hydrothermal field.
KW - 16S rRNA gene
KW - Archaea
KW - Bacteria
KW - Crustal fluids
KW - Deep-sea hydrothermal vent
KW - Island-Arc
KW - Sub-seafloor biosphere
KW - Western Pacific
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U2 - 10.3389/fmicb.2013.00085
DO - 10.3389/fmicb.2013.00085
M3 - Article
C2 - 23626587
AN - SCOPUS:84884261498
SN - 1664-302X
VL - 4
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - APR
ER -