Characterization of Epstein-Barr virus-infected mantle cell lymphoma lines

Zaishun Jin, Norihiro Teramoto, Tadashi Yoshino, Kenzo Takada, Takashi Oka, Kazuhiko Hayashi, Tadaatsu Akagi


4 被引用数 (Scopus)


It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In this study, a mantle cell lymphoma line, SP53, was successfully infected with a recombinant EBV containing a neomycin-resistant gene. The EBV-carrying SP53 cells were obtained by selection using G418. They expressed EBER-1, EBNAs, and LMP1 ; this expression pattern of the EBV genes was similar to that in a lymphoblastoid cell line (LCL). However, proliferation assay showed that the EBV-carrying SP53 cells have a doubling time of 73 h, compared with 57 h of SP53 cells. Transplantation of 108 SP53 cells to nude mice formed tumors in 4 of 10 mice inoculated, but the EBV-carring SP53 cells did not. Unexpectedly, EBV infection reduced the proliferation and tumorigenicity of SP53 cells. However, the EBV-carrying SP53 cells showed higher resistance to apoptosis induced by serum starvation than did the SP53 cells. The inhibition of proliferation and the resistance to apoptosis induced in SP53 cells by EBV infection indicate that this cell line might to some extent provide a model of in vivo EBV reservoir cells.

ジャーナルActa medica Okayama
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)


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