Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song; Hiroyuki Imanaka; Koreyoshi Imamura; Masashi Minoda; Toru Katase; Yukiko Hoshi; Shotaro Yamaguchi; Kazuhiro Nakanishi

doi:10.1271/bbb.110014

Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi

研究成果 › 査読

27 被引用数 (Scopus)

抄録

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

本文言語	English
ページ（範囲）	1194-1197
ページ数	4
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	75
号	6
DOI	https://doi.org/10.1271/bbb.110014
出版ステータス	Published - 2011

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.110014

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abstract = "A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta{\textregistered}. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.",

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AU - Song, Jingyuan

AU - Imanaka, Hiroyuki

AU - Imamura, Koreyoshi

AU - Minoda, Masashi

AU - Katase, Toru

AU - Hoshi, Yukiko

AU - Yamaguchi, Shotaro

AU - Nakanishi, Kazuhiro

PY - 2011

Y1 - 2011

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AB - A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

KW - Bacillus circulans

KW - Galactooligosaccharide

KW - β-galactosidase gene

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

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