@article{5a37d04f99cc403db431d07492c37f00,
title = "Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA",
abstract = "Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.",
author = "Tohru Nakanishi and Yusuke Kimura and Tomoo Tamura and Hiroyuki Ichikawa and Yuichiro Yamaai and Tomosada Sugimoto and Masaharu Takigawa",
note = "Funding Information: We thank Dr. Akira Yamaguchi (Showa University Dental School) for providing Saos-2. We also thank Dr. Kojiro Takahashi and Miss Takako Hattori for useful discussion and Mrs. Etsuko Fujisawa for secretarial assistance. This work was supported in part by Grants-in-Aid for Scienti{\textregistered}c Research from the Ministry of Education, Science, Sports, and Culture of Japan (M.T.) and grants from the Yamada Science Foundation (M.T.), the Sanyo Hohso Foundation (M.T.), the Sagawa Cancer Research Foundation (M.T.), and the Naito Foundation (M.T.), and by funds from Hoechst Japan.",
year = "1997",
month = may,
day = "8",
doi = "10.1006/bbrc.1997.6528",
language = "English",
volume = "234",
pages = "206--210",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",
}