Cytotoxicity of benzyl isothiocyanate in normal renal proximal tubular cells and its modulation by glutathione

Naomi Abe, Masashi Okuhira, Chiharu Tsutsui, Yoshiyuki Murata, Yoshimasa Nakamura

研究成果査読

6 被引用数 (Scopus)

抄録

In the present study, we examined the toxicity of benzyl ITC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC 50value of about 7 μM, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells with oxidative stress. In the presence of 5 mM glutathione (GSH), comparable to physiological levels, the cytotoxicity of BITC-NAC as well as BITC was significantly reduced. Furthermore, the increase in intracellular GSH levels by pretreatment with NAC before the BITC treatment resulted in inhibition of the BITC-induced necrotic events as well as intracellular oxidative stress. These results suggest that GSH is a determinant of cellular resistance against the BITC-mediated and oxidative stress-dependent cytotoxicity in renal proximal tubular cells.

本文言語English
ページ(範囲)1887-1892
ページ数6
ジャーナルJournal of agricultural and food chemistry
60
7
DOI
出版ステータスPublished - 2月 22 2012

ASJC Scopus subject areas

  • 化学一般
  • 農業および生物科学一般

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