Detection of Legionella pneumophila using a nested polymerase chain reaction

F. Higa, M. Koide, T. Shinzato, A. Nakamoto, T. Miyara, M. Gaja, M. Tateyama, I. Owan, N. Kusano, K. Kitsukawa

研究成果査読

1 被引用数 (Scopus)

抄録

We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.

本文言語English
ページ(範囲)1084-1089
ページ数6
ジャーナルKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
66
8
DOI
出版ステータスPublished - 8月 1992
外部発表はい

ASJC Scopus subject areas

  • 医学一般

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