Differential distribution of microtubules in immature osteocytes in vivo

S. A. Murshid, T. Takano-Yamamoto, H. Kamioka

研究成果査読

2 被引用数 (Scopus)

抄録

Objectives: The transition from osteoblasts to osteocytes is associated with dramatic changes in the cytoskeleton. We previously showed that the formation of osteoblast cell processes in 3D culture is microtubule dependent. However, the distribution of microtubules during the transition from osteoblasts to osteocytes in vivo is unknown. In this study, we investigated the distribution of microtubules in osteocytes in vivo. Methods: We observed the microtubules in osteocytes in chick embryonic calvaria via fluorescence staining of microtubules and confocal laser scanning microscopy. Results: Microtubules were observed throughout the cytoplasm in all examined osteoblasts. In immature osteocytes, several cell processes contained microtubules, whereas in mature osteocytes, microtubules were localized only in the cell body. Conclusion: These results suggest that the early arrangement of microtubules may be correlated with the initial development of osteocyte processes.

本文言語English
ページ(範囲)98-101
ページ数4
ジャーナルjournal of oral biosciences
60
4
DOI
出版ステータスPublished - 12月 2018

ASJC Scopus subject areas

  • 医学(その他)
  • 生化学、遺伝学、分子生物学一般
  • 歯学一般

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