We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.
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