TY - JOUR
T1 - Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation
AU - Taga, Masatoki
AU - Tsuchiya, Dai
AU - Murata, Minoru
N1 - Funding Information:
We thank Takashi Tsuge for providing plasmid clones, Hans D. VanEtten and Charlotte R. Bronson for supplying fungal strains. This work was supported in part by a Grant-in-Aid for Special Scientific Research from the Ministry of Agriculture, Forestry and Fisheries of Japan to M.T.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/9/1
Y1 - 2003/9/1
N2 - We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.
AB - We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.
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U2 - 10.1017/S0953756203008244
DO - 10.1017/S0953756203008244
M3 - Article
C2 - 14563127
AN - SCOPUS:0141503490
SN - 0953-7562
VL - 107
SP - 1012
EP - 1020
JO - Mycological Research
JF - Mycological Research
IS - 9
ER -