The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity.
|ジャーナル||Journal of Hard Tissue Biology|
|出版ステータス||Published - 2005|
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