Efficient ethanol production from wheat bran by enzymatic saccharification using commercially available enzyme products and fermentation using bakers' yeast

Mayuko Koreishi, Hiroyuki Imanaka, Koreyoshi Imamura, Masahiro Kariyama, Kazuhiro Nakanishi

研究成果査読

5 被引用数 (Scopus)

抄録

We aimed to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using wheat bran as a biomass. For this purpose, we examined pretreatment conditions of biomass and a combination of commercially available enzyme products for saccharification. When a 3.3% (w/v) wheat bran suspension was pretreated using subcritical water at 140°C with a holding time of 0 h, followed by saccharification at 45°C using a mixture of commercially available Meiselase (composed of cellulase, ce!lobiase/β?- glucosidase, and xylanase) and Novozyme*188 (composed of cellobiase/βS-glucosidase,« amylase, and glucoamylase) at a ratio of 4 to 1 by weight, glucose was released at 10.6 g/l, which corresponded to 90% of the value calculated on the basis of the total amount of glucose-based saccharides contained in the wheat bran. On the other hand, from a pretreated 33% (w/v) wheat bran suspension, 85 g/l glucose was produced, which corresponded to only 72% of the theoretical value, owing to the inhibitory effect of glucose on β?-glucosidase activity. However, when fermentation was carried out at 30°C following 24-h saccharification of the pretreated wheat bran using a mixture of Meiselase and Novozyme*188, the inhibitory effect of glucose was considered to be reduced and as a result, after 21-h fermentation, ethanol was produced at 5.2% (w/v), which corresponded to 88% of the theoretical maximum value.

本文言語English
ページ(範囲)216-223
ページ数8
ジャーナルSeibutsu-kogaku Kaishi
87
5
出版ステータスPublished - 2009

ASJC Scopus subject areas

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー

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