Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1

Hiromitsu Jinno, Masakazu Ueda, Soji Ozawa, Tadashi Ikeda, Kohji Enomoto, Kyriakos Psarras, Masaki Kitajima, Hidenori Yamada, Masaharu Seno

研究成果査読

20 被引用数 (Scopus)

抄録

Recombinant human ribonuclease 1 (RNase 1) was chemically linked to recombinant human epidermal growth factor (EGF). The EGF-RNase conjugate showed dose-dependent cytotoxicity for EGF receptor-overexpressing A431 and TE-8 human squamous carcinoma cells with an IC50 of 2 x 10-7 M and 10-6 M, respectively, whereas the IC50 of RNase alone was almost 10-4M. An unconjugated mixture of EGF and RNase had no greater effect than RNase alone. The conjugate showed no detectable cytotoxicity against EGF receptor-deficient small cell lung cancer cells (H69). Addition to excess EGF in the medium protected A431 cells from the EGF-RNase conjugate cytotoxicity. The cytotoxicity of the EGF-RNase conjugate was positively correlated with the EGF receptor numbers of each cell line. The chimeric toxin composed of only human proteins might be a more useful anti-cancer agent with less immunogenicity than the conventional chimeric toxins.

本文言語English
ページ(範囲)1901-1908
ページ数8
ジャーナルLife Sciences
58
21
DOI
出版ステータスPublished - 4月 19 1996

ASJC Scopus subject areas

  • 薬理学、毒性学および薬学一般
  • 生化学、遺伝学、分子生物学一般

フィンガープリント

「Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル