Genetic alteration of penicillin non-susceptible Streptococcus pneumoniae observed throughout recurrence of acute otitis media detected by amplified fragment length polymorphism analysis

Ken Ichi Sugata, Kunihiro Fukushima, Teruhiro Ogawa, Tomoko Nakashima, Akemi Sugata, Norio Kasai, Mehmet Gunduz, Yasuyoshi Ueki, Kazunori Nishizaki

研究成果査読

1 被引用数 (Scopus)

抄録

The prevalence of penicillin non-susceptible Streptococcus pneumoniae (PNSSP) is increasing among isolates from acute otitis media (AOM). Repeated episodes of antibiotic exposure are a well-known risk factor for the isolation of PNSSP although otitis-prone or recurrent AOM cases frequently require repeated courses of antibiotic treatment. In order to evaluate the chronological alteration of S. pneumoniae during recurrences of AOM, strains of S. pneumoniae were isolated from 11 patients, each of whom had experienced 2-4 episodes of AOM, were examined. Every bacterial specimen obtained from a single episode of recurrent AOM was examined by PCR-based penicillin-binding protein (PBP) assay, serotyping, and amplified fragment length polymorphism (AFLP), then compared to other samples from the same case. Two cases (18.2%) showed strain diversity during repeated antibiotic treatments by serotyping or PBP-assay. By AFLP analysis, 6 cases (54.5%) demonstrated heterogeneous strains during recurrent AOM. Clonal survivors of previous episodes of AOM were not always the cause of subsequent episodes of AOM, even in otitis-prone cases.

本文言語English
ページ(範囲)167-174
ページ数8
ジャーナルActa medica Okayama
55
3
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学一般

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