TY - JOUR
T1 - Genetic backgrounds and redox conditions influence morphological characteristics and cell differentiation of osteoclasts in mice
AU - Narahara, Shun
AU - Matsushima, Haruna
AU - Sakai, Eiko
AU - Fukuma, Yutaka
AU - Nishishita, Kazuhisa
AU - Okamoto, Kuniaki
AU - Tsukuba, Takayuki
N1 - Funding Information:
Acknowledgments This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (E.S, T.T).
PY - 2012/4
Y1 - 2012/4
N2 - Osteoclasts (OCLs) are multinucleated giant cells and are formed by the fusion of mononuclear progenitors of monocyte/macrophage lineage. It is known that macrophages derived from different genetic backgrounds exhibit quite distinct characteristics of immune responses. However, it is unknown whether OCLs from different genetic backgrounds show distinct characteristics. In this study, we showed that bone-marrow macrophages (BMMs) derived from C57BL/6, BALB/c and ddY mice exhibited considerably distinct morphological characteristics and cell differentiation into OCLs. The differentiation of BMMs into OCLs was comparatively quicker in the C57BL/6 and ddY mice, while that of BALB/c mice was rather slow. Morphologically, ddY OCLs showed a giant cell with a round shape, C57BL/6 OCLs were of a moderate size with many protrusions and BALB/c OCLs had the smallest size with fewer nuclei. The intracellular signaling of differentiation and expression levels of marker proteins of OCLs were different in the respective strains. Treatment of BMMs from the three different strains with the reducing agent N-acetylcysteine (NAC) or with the oxidation agent hydrogen peroxide (H 2O 2) induced changes in the shape and sizes of the cells and caused distinct patterns of cell differentiation and survival. Thus, genetic backgrounds and redox conditions regulate the morphological characteristics and cell differentiation of OCLs.
AB - Osteoclasts (OCLs) are multinucleated giant cells and are formed by the fusion of mononuclear progenitors of monocyte/macrophage lineage. It is known that macrophages derived from different genetic backgrounds exhibit quite distinct characteristics of immune responses. However, it is unknown whether OCLs from different genetic backgrounds show distinct characteristics. In this study, we showed that bone-marrow macrophages (BMMs) derived from C57BL/6, BALB/c and ddY mice exhibited considerably distinct morphological characteristics and cell differentiation into OCLs. The differentiation of BMMs into OCLs was comparatively quicker in the C57BL/6 and ddY mice, while that of BALB/c mice was rather slow. Morphologically, ddY OCLs showed a giant cell with a round shape, C57BL/6 OCLs were of a moderate size with many protrusions and BALB/c OCLs had the smallest size with fewer nuclei. The intracellular signaling of differentiation and expression levels of marker proteins of OCLs were different in the respective strains. Treatment of BMMs from the three different strains with the reducing agent N-acetylcysteine (NAC) or with the oxidation agent hydrogen peroxide (H 2O 2) induced changes in the shape and sizes of the cells and caused distinct patterns of cell differentiation and survival. Thus, genetic backgrounds and redox conditions regulate the morphological characteristics and cell differentiation of OCLs.
KW - BALB/c
KW - C57BL/6
KW - Differentiation
KW - Osteoclasts
KW - ddY
UR - http://www.scopus.com/inward/record.url?scp=84859445154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859445154&partnerID=8YFLogxK
U2 - 10.1007/s00441-012-1325-8
DO - 10.1007/s00441-012-1325-8
M3 - Article
C2 - 22350845
AN - SCOPUS:84859445154
SN - 0302-766X
VL - 348
SP - 81
EP - 94
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 1
ER -