Genome-wide Loss of Heterozygosity Analysis in Head and Neck Squamous Cell Carcinomas

Levent Beder, Mehmet Gunduz, Kazunori Nishizaki, Kenji Shimizu, Noriyuki Nagai, Keihachi Fukunishi, Yumiko Hiura, Noriaki Matsumoto

研究成果査読

1 被引用数 (Scopus)

抄録

Identifying the tumor suppressor gene (TSG) loci by genomic studies is an important step to uncover the molecular mechanisms involved in HNSCC pathogenesis. We therefore performed comprehensive analyses on loss of heterozygosity (LOH) using a genome-wide panel of 191 microsatellite markers in 22 HNSCC samples. We found 53 markers with significantly high LOH (>30%) on 21 chromosomal arms, the highest values of those were observed on 3p, 9p, 13q, 15q, and 17p, corresponding to D3S2432 (67%), D9S921-D9S925 (67%) and GATA62F03 (86%), D13S1493 (60%), D15S211 (62%) and D17S1353 (88%), respectively. Fifteen hot spots of LOH were defined in 13 chromosomal arms reported previously in HNSCCs. Furthermore, we identified 5 novel hot spots of LOH on 3 chromosomal arms in HNSCC at 2q33 (D2S1384), 2q37 (D2S125), 8q12-13 (D8S1136), 8q24 (D8S1128) and 15q21 (D15S211). In conclusion, our comprehensive allelotype analyses have unveiled and confirmed a total of 20 possible TSG loci that could be involved in the development of HNSCC. These results provide useful clues for identification of putative TSGs involved in HNSCC by fine mapping of the suspected regions.

本文言語English
ページ(範囲)229-231
ページ数3
ジャーナルJournal of Hard Tissue Biology
14
2
DOI
出版ステータスPublished - 1月 2005
外部発表はい

ASJC Scopus subject areas

  • 医学(その他)
  • 生化学
  • 生体材料
  • 整形外科およびスポーツ医学
  • 歯学一般
  • 細胞生物学

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