@inproceedings{2c148a6f3b7a4decb15314512a385a9c,
title = "Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay",
abstract = "This paper describes a quantitative immunoassay chip harnessing EOF (electroosmotic flow) in glass fiber sheet. The chip consists of a glass fiber sheet-based channel covered with PMMA (polymethylmethacrylate) frames and antibody-immobilized microbeads at the observation spot (Fig. 1). Applying EOF, another labeled-antibody and rinsing buffer sequentially migrate through the antigen-antibody complex on the beads and stain the beads depending on the sample (antigen) concentration. We demonstrated a quantitative assay of insulin and succeeded in determining the concentration in 20 min. The developed chip would provide a rapid, low cost and contamination-free assay system.",
keywords = "Electroosmotic flow, Glass fiber sheet, Immunoassay",
author = "Yuriko Oyama and Toshihisa Osaki and Koki Kamiya and Ryuji Kawano and Tsutomu Honjoh and Haruki Shibata and Toru Ide and Shoji Takeuchi",
year = "2012",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "1957--1959",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}