Glutathione transport systems of the budding yeast saccharomyces cerevisiae

Tsuyoshi Miyake, Toshiya Hazu, Sayaka Yoshida, Munetoshi Kanayama, Ken Ichi Tomochika, Sumio Shinoda, Bun Ichiro Ono

研究成果査読

32 被引用数 (Scopus)

抄録

The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport systems. While one with high affinity (GSH-P1; KT=0.045 mM) was regulated, the other with low affinity (GSH-P2; KT>2 mM) was not. GSH-P1 was highly specific to glutathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, ρ isolates had only about one half of the glutathione transport activity of the original (ρ +) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, re.

本文言語English
ページ(範囲)1858-1864
ページ数7
ジャーナルBioscience, Biotechnology and Biochemistry
62
10
DOI
出版ステータスPublished - 1月 1 1998

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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