TY - JOUR
T1 - Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor
AU - Takebe, T.
AU - Kobayashi, S.
AU - Kan, H.
AU - Suzuki, H.
AU - Yabuki, Y.
AU - Mizuno, M.
AU - Adegawa, T.
AU - Yoshioka, T.
AU - Tanaka, J.
AU - Maegawa, J.
AU - Taniguchi, H.
N1 - Funding Information:
This work was supported by a Grant-in-aid for Developmental Scientific Research, The Ministry of Education, Science and Culture (No. 20592101 ) and Health and Labour Sciences Research Grants Research on intractable disease (No. 2011-164 ). This work was also supported by the grant for Research and Development Project (No. 19-15 ) of Yokohama City University, Japan.
PY - 2012/5
Y1 - 2012/5
N2 - Transplantation of bioengineered elastic cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D elastic cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and elastic fibers, which are unique extracellular matrices of elastic cartilage. Thus, human CPCs formed elastic cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of elastic cartilage in vitro.
AB - Transplantation of bioengineered elastic cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D elastic cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and elastic fibers, which are unique extracellular matrices of elastic cartilage. Thus, human CPCs formed elastic cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of elastic cartilage in vitro.
UR - https://www.scopus.com/pages/publications/84860745735
UR - https://www.scopus.com/pages/publications/84860745735#tab=citedBy
U2 - 10.1016/j.transproceed.2012.03.038
DO - 10.1016/j.transproceed.2012.03.038
M3 - Article
C2 - 22564652
AN - SCOPUS:84860745735
SN - 0041-1345
VL - 44
SP - 1158
EP - 1161
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 4
ER -