Illumination accelerates the decay of the O-intermediate of pharaonis phoborhodopsin (sensory rhodopsin II)

Masayuki Iwamoto, Yuki Sudo, Kazumi Shimono, Naoki Kamo

研究成果査読

1 被引用数 (Scopus)

抄録

pharaonis phoborhodopsin (ppR, also called pharaonis sensory rhodopsin II [psRII]) is a member of the archaeal rhodopsin family and acts as a repellent phototaxis receptor of Natronobacterium pharaonis. Upon illumination, ppR is excited and undergoes a linear cyclic photoreaction, namely, a photocycle that constitutes photointermediates such as M- and O-intermediates (ppRM and ppRO, respectively). Under a constant background illumination (>600 nm) that irradiates ppRO, the decay rate of the flash-induced ppRO increased with an increase in the background light intensity, indicating the photoreactivity of ppRO. Azide did not influence the light-accelerated ppRO decay, but the time required for the cycle to be completed became shortened in an azide concentration-dependent manner because of acceleration of ppRM decay. Hence, the turnover rate of photocycling increased appreciably in the presence of both the background illumination and the azide. The observation reported previously (Schmies, G. et al. 2000, Biophys. J. 78:967-976) is discussed in connection with the present observations.

本文言語English
ページ(範囲)462-466
ページ数5
ジャーナルPhotochemistry and Photobiology
76
4
DOI
出版ステータスPublished - 10月 1 2002
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学

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