TY - JOUR
T1 - Immobilized liposome chromatography for refolding and purification of protein
AU - Yoshimoto, Makoto
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Kuboi, Ryoichi
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research (No. 08555183 and No. 09650828) of The Ministry of Education, Science, Sports and Culture of Japan. M.Y. acknowledges the financial support for this work by the fellowship of the Japan Society for the Promotion of Science for the Japan Junior Scientists.
PY - 2000/6/23
Y1 - 2000/6/23
N2 - Small unilamellar liposomes were utilized as a kind of aqueous two-phase system and artificial chaperone which specifically recognize protein conformation with fluctuated structure. Liposomes showed highly selective binding ability to conformationally changed proteins treated with various concentrations of guanidinium hydrochloride, as evaluated by immobilized liposome chromatography (ILC). In refolding of proteins, liposomes bound to refolding intermediate of proteins and prevented them from forming intermolecular aggregates. Refolding of bovine carbonic anhydrase, lysozyme and ribonuclease A was significantly improved in the presence of liposomes. Furthermore, by utilizing ILC, refolding of proteins was also successfully and simply carried out with considerable high reactivation yield.
AB - Small unilamellar liposomes were utilized as a kind of aqueous two-phase system and artificial chaperone which specifically recognize protein conformation with fluctuated structure. Liposomes showed highly selective binding ability to conformationally changed proteins treated with various concentrations of guanidinium hydrochloride, as evaluated by immobilized liposome chromatography (ILC). In refolding of proteins, liposomes bound to refolding intermediate of proteins and prevented them from forming intermolecular aggregates. Refolding of bovine carbonic anhydrase, lysozyme and ribonuclease A was significantly improved in the presence of liposomes. Furthermore, by utilizing ILC, refolding of proteins was also successfully and simply carried out with considerable high reactivation yield.
KW - Immobilized liposome chromatography
KW - Lipid bilayer membranes
KW - Protein refolding
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U2 - 10.1016/S0378-4347(00)00051-7
DO - 10.1016/S0378-4347(00)00051-7
M3 - Article
C2 - 10942276
AN - SCOPUS:0034705747
SN - 0378-4347
VL - 743
SP - 93
EP - 99
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1-2
ER -