Immunological Detection of F1-ATPase γ-Subunit in Rat Liver Peroxisomes

Tsuneo Imanaka; Yu’ichi Shiina; Yoshinori Moriyama; Shoji Ohkuma; Tatsuya Takano

doi:10.1006/bbrc.1993.2147

Immunological Detection of F₁-ATPase γ-Subunit in Rat Liver Peroxisomes

Tsuneo Imanaka, Yu’ichi Shiina, Yoshinori Moriyama, Shoji Ohkuma, Tatsuya Takano

薬学部

研究成果 › 査読

2 被引用数 (Scopus)

抄録

The γ-subunit of F₁-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F₁-ATPase. Other subunits of F₁-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F₁-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F₁-ATPase is a constituent of peroxisomes in rat liver.

本文言語	English
ページ（範囲）	1027-1034
ページ数	8
ジャーナル	Biochemical and Biophysical Research Communications
巻	195
号	2
DOI	https://doi.org/10.1006/bbrc.1993.2147
出版ステータス	Published - 9月 15 1993

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

文献へのアクセス

10.1006/bbrc.1993.2147

他のファイルとリンク

引用スタイル

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title = "Immunological Detection of F1-ATPase γ-Subunit in Rat Liver Peroxisomes",

abstract = "The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.",

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AU - Imanaka, Tsuneo

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AU - Ohkuma, Shoji

AU - Takano, Tatsuya

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AB - The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.

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