TY - JOUR
T1 - Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans
AU - Watanabe, Naoshi
AU - Morimatsu, Masatoshi
AU - Fujita, Ayano
AU - Teranishi, Mika
AU - Sudevan, Surabhi
AU - Watanabe, Masaru
AU - Iwasa, Hiroaki
AU - Hata, Yutaka
AU - Kagi, Hiroyuki
AU - Nishiyama, Masayoshi
AU - Naruse, Keiji
AU - Higashitani, Atsushi
N1 - Funding Information:
We thank Professor Masahiro Sokabe for his discussions and comments on the manuscript. The Caenorhabditis elegans strains used in this study were provided by the Caenorhabditis Genetics Center. The Center was funded by the Office of Research Infrastructure Programs , National Institutes of Health ( P40 OD010440 ). This work was funded in part by the Japan Society for the Promotion of Science ( KAKENHI grant numbers JP15H05936 , JP15H05937 , JP18K12033 , JP19H04679 and JP19H02566 ), and by the Advanced Research and Development Programs for Medical Innovation (AMED- CREST 1681430 ). Appendix A
Funding Information:
We thank Professor Masahiro Sokabe for his discussions and comments on the manuscript. The Caenorhabditis elegans strains used in this study were provided by the Caenorhabditis Genetics Center. The Center was funded by the Office of Research Infrastructure Programs, National Institutes of Health (P40 OD010440). This work was funded in part by the Japan Society for the Promotion of Science (KAKENHI grant numbers JP15H05936, JP15H05937, JP18K12033, JP19H04679 and JP19H02566), and by the Advanced Research and Development Programs for Medical Innovation (AMED-CREST 1681430).
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/3/19
Y1 - 2020/3/19
N2 - Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.
AB - Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.
KW - Ageing
KW - Longevity
KW - Mechanical stimulation
KW - Mega pascal
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=85077919797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077919797&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2020.01.047
DO - 10.1016/j.bbrc.2020.01.047
M3 - Article
C2 - 31954516
AN - SCOPUS:85077919797
SN - 0006-291X
VL - 523
SP - 853
EP - 858
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -