Insertion of a suicide gene into an immortalized human hepatocyte cell line

N. Kobayashi, H. Noguchi, T. Totsugawa, T. Watanabe, T. Matsumura, T. Fujiwara, M. Miyazaki, K. Fukaya, M. Namba, N. Tanaka


2 被引用数 (Scopus)


For developing a bioartificial liver (BAL) device, an attractive alternative to the primary human hepatocytes would be the use of highly differentiated immortalized human hepatocytes with a safeguard. To test the feasibility, the primary human hepatocytes were immortalized by a plasmid SV3neo encoding simian virus 40 large T antigen (SV40Tag) gene. A highly differentiated hepatocyte line OUMS-29 was established. A suicide gene of herpes simplex virus-thymidine kinase (HSV-TK) was retrovirally introduced into OUMS-29 cells as a safeguard for clinical application. One of the resulting HSV-TK-positive cell lines, OUMS-29/ tk, grew in chemically defined serum-free medium with the gene expression of differentiated liver functions. OUMS-29/tk cells were 100 times more sensitive to ganciclovir compared with unmodified OUMS-29 cells in in vitro experiments. We have established a tightly regulated immortalized human hepatocyte cell line. Essentially unlimited availability of OUMS-29/tk cells may be clinically useful for BAL therapy.

ジャーナルCell Transplantation
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 生体医工学
  • 細胞生物学
  • 移植


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