Internal anion binding site and membrane potential dominate the regulation of proton pumping by the chromaffin granule ATPase

Y. Moriyama, N. Nelson

研究成果査読

20 被引用数 (Scopus)

抄録

Effects of anions and membrane potential on the reconstituted proton pump from chromaffin granules were investigated. When acetate was present inside of the vesicles, ATP-dependent proton uptake was absolutely dependent on external chloride. Without external chloride, however, substantial proton uptake was observed when chloride or sulfate was present inside of the vesicles. Inside negative membrane potential drove ATP-dependent proton uptake regardless of the anion species present inside or outside of the vesicles. It is concluded that the internal anion binding site and membrane potential regulate the proton pumping activity of the ATPase.

本文言語English
ページ(範囲)140-144
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
149
1
DOI
出版ステータスPublished - 11月 30 1987

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Internal anion binding site and membrane potential dominate the regulation of proton pumping by the chromaffin granule ATPase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル