Lats2 is an essential mitotic regulator required for the coordination of cell division

Norikazu Yabuta, Nobuhiro Okada, Akihiko Ito, Toshiya Hosomi, Souichi Nishihara, Yuya Sasayama, Azumi Fujimori, Daisuke Okuzaki, Hanjun Zhao, Masahito Ikawa, Masaru Okabe, Hiroshi Nojima

研究成果査読

134 被引用数 (Scopus)

抄録

Tumor suppressor Lats2 is a member of the conserved Dbf2 kinase family. It localizes to the centrosome and has been implicated in regulation of the cell cycle and apoptosis. However, the in vivo function of this kinase remains unclear. Here, we show that complete disruption of the gene encoding Lats2 in mice causes developmental defects in the nervous system and embryonic lethality. Furthermore, mutant cells derived from total LATS2-knock-out embryos exhibit mitotic defects including centrosome fragmentation and cytokinesis defects, followed by nuclear enlargement and multinucleation. We show that the Mob1 family, a regulator of mitotic exit, associates with Lats2 to induce its activation. We also show that the complete LATS2-knock-out cells exhibit an acceleration of exit from mitosis and marked down-regulation of critical mitotic regulators. These results suggest that Lats2 plays an essential mitotic role in coordinating accurate cytokinesis completion, governing the stabilization of other mitotic regulators.

本文言語English
ページ(範囲)19259-19271
ページ数13
ジャーナルJournal of Biological Chemistry
282
26
DOI
出版ステータスPublished - 6月 29 2007
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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