TY - JOUR
T1 - Molecular Anatomy of a Trafficking Organelle
AU - Takamori, Shigeo
AU - Holt, Matthew
AU - Stenius, Katinka
AU - Lemke, Edward A.
AU - Grønborg, Mads
AU - Riedel, Dietmar
AU - Urlaub, Henning
AU - Schenck, Stephan
AU - Brügger, Britta
AU - Ringler, Philippe
AU - Müller, Shirley A.
AU - Rammner, Burkhard
AU - Gräter, Frauke
AU - Hub, Jochen S.
AU - De Groot, Bert L.
AU - Mieskes, Gottfried
AU - Moriyama, Yoshinori
AU - Klingauf, Jürgen
AU - Grubmüller, Helmut
AU - Heuser, John
AU - Wieland, Felix
AU - Jahn, Reinhard
N1 - Funding Information:
The authors are indebted to the following for reagents, help, and/or advice: A. Stein, M. Druminski, S.O. Rizzoli, and M. Raabe (Göttingen, Germany); A. Engel, R. Buerki, and F. Erne-Brand (Basel, Switzerland); V. Krzyzanek (Münster, Germany); and K.M. Harris (Augusta, GA, USA). We apologize to all colleagues whose work, although relevant, could not be quoted due to space limitations. S.T. was supported by JSPS in part during this work. M.G. was supported by the Danish Agency for Science, Technology and Innovation (DASTI). S.A.M. was supported by the Maurice E. Müller Foundation of Switzerland and the Swiss National Foundation (grant number 3100-059415). R.J. acknowledges generous support from the Gottfried-Wilhelm Leibniz Program of the DFG and Fonds der Chemischen Industrie.
PY - 2006/11/17
Y1 - 2006/11/17
N2 - Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
AB - Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
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U2 - 10.1016/j.cell.2006.10.030
DO - 10.1016/j.cell.2006.10.030
M3 - Article
C2 - 17110340
AN - SCOPUS:33750805030
SN - 0092-8674
VL - 127
SP - 831
EP - 846
JO - Cell
JF - Cell
IS - 4
ER -