Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

Nobuaki Okumura; Masanori Ikeda; Shinya Satoh; Hiromichi Dansako; Masaya Sugiyama; Masashi Mizokami; Nobuyuki Kato

doi:10.1016/j.febslet.2015.03.022

Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

Nobuaki Okumura, Masanori Ikeda, Shinya Satoh, Hiromichi Dansako, Masaya Sugiyama, Masashi Mizokami, Nobuyuki Kato

研究成果 › 査読

7 被引用数 (Scopus)

抄録

Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

本文言語	English
ページ（範囲）	1112-1118
ページ数	7
ジャーナル	FEBS Letters
巻	589
号	10
DOI	https://doi.org/10.1016/j.febslet.2015.03.022
出版ステータス	Published - 4月 28 2015

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.febslet.2015.03.022

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title = "Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells",

abstract = "Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.",

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author = "Nobuaki Okumura and Masanori Ikeda and Shinya Satoh and Hiromichi Dansako and Masaya Sugiyama and Masashi Mizokami and Nobuyuki Kato",

note = "Funding Information: We also thank Masayo Takemoto, Narumi Yamane, and Takashi Nakamura for their technical assistance. This work was supported by Grants-in-Aid for Research on Hepatitis from the Ministry of Health, Labor, and Welfare of Japan . Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

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AU - Okumura, Nobuaki

AU - Ikeda, Masanori

AU - Satoh, Shinya

AU - Dansako, Hiromichi

AU - Sugiyama, Masaya

AU - Mizokami, Masashi

AU - Kato, Nobuyuki

N1 - Funding Information: We also thank Masayo Takemoto, Narumi Yamane, and Takashi Nakamura for their technical assistance. This work was supported by Grants-in-Aid for Research on Hepatitis from the Ministry of Health, Labor, and Welfare of Japan . Publisher Copyright: © 2015 Federation of European Biochemical Societies.

PY - 2015/4/28

Y1 - 2015/4/28

N2 - Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

AB - Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

KW - FOXA1

KW - FOXA2

KW - FOXA3

KW - HNF3

KW - Hepatitis B virus

KW - Hepatitis B virus replication

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JO - FEBS Letters

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Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

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ASJC Scopus subject areas

UN SDG

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