Nuclease-sensitive binding of an Actinobacillus actinomycetemcomitans leukotoxin to the bacterial cell surface

H. Ohta, K. Kato, S. Kokeguchi, H. Hara, K. Fukui, Y. Murayama

研究成果査読

43 被引用数 (Scopus)

抄録

A leukotoxin of Actinobacillus actinomycetemcomitans 301-b was solubilized from cell-associated membrane vesicles by treatment with externally added DNase and RNase and was further purified by a procedure which included ammonium sulfate fractionation, gel filtration chromatography, and ion- exchange chromatography. The purified toxin had a molecular mass of 113,000 Da by sodium dodecyl sulfate-polyacrylamide gel eletrophoresis and a high isoelectric point (approximately 8.8). From these characteristics, it was to be expected that the membrane vesicle toxin was almost identical to the leukotoxin extracted with polymyxin B in an earlier study (C.-C. Tsai, B. J. Shenker, J. M. DiRienzo, D. Malamud, and N. S. Taichman, Infect. Immun. 43:700-705, 1984). The treatment with DNase and RNase was also highly effective for solubilizing the leukotoxin directly from whole cells, suggesting that the toxin is secreted extracellularly but retained in nucleic acids on the outermost surface of bacterial cells.

本文言語English
ページ(範囲)4599-4605
ページ数7
ジャーナルInfection and Immunity
59
12
出版ステータスPublished - 1991
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 微生物学
  • 免疫学
  • 感染症

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