Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen

Yoshinobu Kimura, Maiko Kamamoto, Megumi Maeda, Mitsuhiro Okano, Minehiko Yokoyama, Kosuke Kino

研究成果査読

22 被引用数 (Scopus)

抄録

We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.

本文言語English
ページ(範囲)137-144
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
69
1
DOI
出版ステータスPublished - 1月 2005

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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