TY - JOUR
T1 - Orexin A and B in the rat superior salivatory nucleus
AU - Sato, Tadasu
AU - Yajima, Takehiro
AU - Fujita, Masako
AU - Kobashi, Motoi
AU - Ichikawa, Hiroyuki
AU - Yoshida, Ryusuke
AU - Mitoh, Yoshihiro
N1 - Funding Information:
We thank the Biomedical Research Core of Tohoku University Graduate School of Medicine (Japan) for technical support. This study was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grants (Nos. 25462887 and 18K11022).
Funding Information:
We thank the Biomedical Research Core of Tohoku University Graduate School of Medicine (Japan) for technical support. This study was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grants (Nos. 25462887 and 18K11022 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11
Y1 - 2020/11
N2 - Orexin (OX), which regulates sleep and wakefulness and feeding behaviors has 2 isoforms, orexin-A and -B (OXA and OXB). In this study, the distribution of OXA and OXB was examined in the rat superior salivatory nucleus (SSN) using retrograde tracing and immunohistochemical and methods. OXA- and OXB-immunoreactive (-ir) nerve fibers were seen throughout the SSN. These nerve fibers surrounded SSN neurons retrogradely labeled with Fast blue (FB) from the corda-lingual nerve. FB-positive neurons had pericellular OXA- (47.5%) and OXB-ir (49.0%) nerve fibers. Immunohistochemistry for OX receptors also demonstrated the presence of OX1R and OX2R in FB-positive SSN neurons. The majority of FB-positive SSN neurons contained OX1R- (69.7%) or OX2R-immunoreactivity (57.8%). These neurons had small and medium-sized cell bodies. In addition, half of FB-positive SSN neurons which were immunoreactive for OX1R (47.0%) and OX2R (52.2%) had pericellular OXA- and OXB-ir nerve fibers, respectively. Co-expression of OX1R- and OX2R was common in FB-positive SSN neurons. The present study suggests a possibility that OXs regulate the activity of SSN neurons through OX receptors.
AB - Orexin (OX), which regulates sleep and wakefulness and feeding behaviors has 2 isoforms, orexin-A and -B (OXA and OXB). In this study, the distribution of OXA and OXB was examined in the rat superior salivatory nucleus (SSN) using retrograde tracing and immunohistochemical and methods. OXA- and OXB-immunoreactive (-ir) nerve fibers were seen throughout the SSN. These nerve fibers surrounded SSN neurons retrogradely labeled with Fast blue (FB) from the corda-lingual nerve. FB-positive neurons had pericellular OXA- (47.5%) and OXB-ir (49.0%) nerve fibers. Immunohistochemistry for OX receptors also demonstrated the presence of OX1R and OX2R in FB-positive SSN neurons. The majority of FB-positive SSN neurons contained OX1R- (69.7%) or OX2R-immunoreactivity (57.8%). These neurons had small and medium-sized cell bodies. In addition, half of FB-positive SSN neurons which were immunoreactive for OX1R (47.0%) and OX2R (52.2%) had pericellular OXA- and OXB-ir nerve fibers, respectively. Co-expression of OX1R- and OX2R was common in FB-positive SSN neurons. The present study suggests a possibility that OXs regulate the activity of SSN neurons through OX receptors.
KW - Chorda-lingual nerve
KW - Immunohistochemistry
KW - Orexin
KW - Orexin receptor
KW - Preganglionic neuron
KW - Superior salivatory nucleus
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U2 - 10.1016/j.autneu.2020.102712
DO - 10.1016/j.autneu.2020.102712
M3 - Article
C2 - 32721850
AN - SCOPUS:85088433571
SN - 1566-0702
VL - 228
JO - Journal of the Autonomic Nervous System
JF - Journal of the Autonomic Nervous System
M1 - 102712
ER -