TY - JOUR
T1 - Organellar Glue
T2 - A Molecular Tool to Artificially Control Chloroplast-Chloroplast Interactions
AU - Ichikawa, Shintaro
AU - Kato, Shota
AU - Fujii, Yuta
AU - Ishikawa, Kazuya
AU - Numata, Keiji
AU - Kodama, Yutaka
N1 - Funding Information:
The authors thank Ms. Rieko Saijo, Ms. Noriko Hamashima, Ms. Yuka Ogasawara, and Dr. Shoko Tsuboyama (Utsunomiya University) for technical assistance. This work was supported by the Japan Science and Technology Agency Exploratory Research for Advanced Technology (JST-ERATO; Grant Number JPMJER1602). Yutaka Kodama thanks the late Prof. Chang-Deng Hu (Purdue University), who passed away on September 1, 2022, for the initial development of BiFC technology (Hu et al. Mol. Cell 2002 , 9, 789−798) and his guidance to date. May he rest in peace.
Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.
PY - 2022/10/21
Y1 - 2022/10/21
N2 - Organelles can physically interact to facilitate various cellular processes such as metabolite exchange. Artificially regulating these interactions represents a promising approach for synthetic biology. Here, we artificially controlled chloroplast-chloroplast interactions in living plant cells with our organelle glue (ORGL) technique, which is based on reconstitution of a split fluorescent protein. We simultaneously targeted N-terminal and C-terminal fragments of a fluorescent protein to the chloroplast outer envelope membrane or cytosol, respectively, which induced chloroplast-chloroplast interactions. The cytosolic C-terminal fragment likely functions as a bridge between two N-terminal fragments, thereby bringing the chloroplasts in close proximity to interact. We modulated the frequency of chloroplast-chloroplast interactions by altering the ratio of N- A nd C-terminal fragments. We conclude that the ORGL technique can successfully control chloroplast-chloroplast interactions in plants, providing a proof of concept for the artificial regulation of organelle interactions in living cells.
AB - Organelles can physically interact to facilitate various cellular processes such as metabolite exchange. Artificially regulating these interactions represents a promising approach for synthetic biology. Here, we artificially controlled chloroplast-chloroplast interactions in living plant cells with our organelle glue (ORGL) technique, which is based on reconstitution of a split fluorescent protein. We simultaneously targeted N-terminal and C-terminal fragments of a fluorescent protein to the chloroplast outer envelope membrane or cytosol, respectively, which induced chloroplast-chloroplast interactions. The cytosolic C-terminal fragment likely functions as a bridge between two N-terminal fragments, thereby bringing the chloroplasts in close proximity to interact. We modulated the frequency of chloroplast-chloroplast interactions by altering the ratio of N- A nd C-terminal fragments. We conclude that the ORGL technique can successfully control chloroplast-chloroplast interactions in plants, providing a proof of concept for the artificial regulation of organelle interactions in living cells.
KW - bimolecular fluorescence complementation (BiFC)
KW - chloroplasts
KW - Egeria densa
KW - mitochondria
KW - organelle interaction
KW - peroxisome
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U2 - 10.1021/acssynbio.2c00367
DO - 10.1021/acssynbio.2c00367
M3 - Article
C2 - 36178266
AN - SCOPUS:85139437413
SN - 2161-5063
VL - 11
SP - 3190
EP - 3197
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 10
ER -